GE Healthcare Life Sciences - Gel Filtration, Principles and Methods Gel filtration is a robust technique that is well suited to handling biomolecules that are sensitive to changes in pH, concentration of metal ions or co-factors and harsh environmental conditions. Separations can be performed in the presence of essential ions or cofactors, detergents, urea, guanidine hydrochloride, at high or low ionic strength, at 37 °C or in the cold room according to the requirements of the experiment.This handbook describes the use of gel filtration for the purification and separation with biomoleculesfiltrationgelhandlingrobustsensitivesuitedtechnique